# Overview
Instrumentation should be affordable, reliable, and easy to use to enable as many people as possible to contribute to research. The instruments developed to work with PhotosynQ try to bridge this chasm between what is currently available and the for mentioned goals.
# MultispeQ v2.5 and v2.0
MultispeQ v2.5 and v2.0 share the same setup, operation, application, Protocol, Macro, QC, cleaning, and maintenance workflows. Both combine PAM-style chlorophyll fluorescence, absorbance-based spectroscopy, environmental sensing, and PhotosynQ-connected data collection. Version 2.5 introduces a limited set of updated optical components without changing normal operation.
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# CaliQ
The CaliQ is a calibrated PAR (Photosynthetically Active Radiation) sensor and a calibrated white light source to calibrate a MultispeQ (v1.0 and v2.0).
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Note
The CaliQ requires a compatible MultispeQ to be operated.
# Legacy Instruments
The following instruments are retained for operators maintaining earlier studies and hardware. They are not the current MultispeQ generation.
# MultispeQ v1.0
The MultispeQ v1.0 is based on the experiences we gained from our development of the beta version. It measures fluorescence base parameters like qL, qP, PhiII, LEF, NPQ, and absorbance based parameters like vH⁺, gH⁺, ECSt, and relative chlorophyll content. It can collect environmental parameters like temperature, relative humidity, barometric pressure, leaf temperature, light intensity, cardinal direction, and tilt.
The signal quality has been increased to allow absorbance based measurements like the electrochromic shift in the field within a reasonable time. The ergonomics and the leaf clamping has been improved based on experiences in the field.
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WARNING
This version of the Instrument is no longer available.
# MultispeQ beta
This instrument was the first developed as a proof of concept to show it is possible to measure photosynthetic parameters with an easy to use, field deployable instrument. This includes parameters like fluorescence base parameters like qL, qP, ΦII, LEF, NPQ, and absorbance based parameters like vH⁺, gH⁺, ECSt, and relative chlorophyll content. On top, it can collect environmental parameters like temperature, relative humidity, light intensity, and CO₂ concentration.
It has been a reliable workhorse for the last two years and is still used in several studies. For more details and our validation of the instrument, please read the publication (open access).
Reference
Kuhlgert, S., Austic, G., Zegarac, R. Osei-Bonsu, I.,Hoh, D., Chilvers, M. I., et al. (2016). MultispeQ Beta: a tool for large-scale plant phenotyping connected to the open PhotosynQ network. R. Soc. Open Sci. 3, 160592. doi:10.1098/rsos.160592 (opens new window).
WARNING
This version of the Instrument is no longer available and no longer supported.