# MultispeQ v2.0

# Getting Started

  • Charging: Before using your MultispeQ, charge it for 8 hours using the supplied micro-USB cable*. When plugged in, the small green LED next to the micro-USB port lights up. Once the instrument is fully charged, the light will turn off.
  • Turn On: Press and hold the power/reset button in the rear for 5 seconds.
  • Software: Go to https://photosynq.com/software (opens new window) and download the Android app or the Desktop app.

* Power supply not included. Use a computer or standard USB power supply. Charging time depends on the power output of the power supply.

# About

MultispeQ v2.0 side and back view

# Leaf Clamp Button

Push down the leaf clamp button to open the leaf clamp. Push and release with an even motion to prevent it from snapping back. In case the opening and closing feels rough, a small amount of lithium grease can be used to make the motion smoother.

# PAR Sensor

The PAR sensor is located on top of the instrument. When taking measurements, depending on the measurement protocol, the light intensity measured will be replicated inside the instrument. Make sure the PAR sensor is clean and not covered or shaded to prevent altering the actual intensity.

# Seal

The Nitrile seal around the light guide on the main body and the clamp is ensuring the area measured is sealed from outside light.

# Light Guide

The light guides are made from acrylic. Make sure it is clean and prevent it clamping hard rough materials which can cause scratches.


You may need to clean the light guides occasionally. Gently clean the light guides with a damp, soft cloth.


Do not submerge in water, do not use solvents or detergents and do not use abrasive or rough cloths to clean the light guides.

# IR Contactless Temperature

The leaf temperature can be measured with the IR Contactless Temperature. When taking a measurement, make sure that the leaf is covering the IR sensor. The sensor is inside a little groove and not flush with the clamp surface. The leaf or material should not touch the the sensor, since it can alter the measured temperature reading.

# Temperature & rel. Humidity Vent

The two vents allow air to be exchanged inside the Instrument. There are two sensors inside the clamp, one is close to the vents and the light guide while the second one is located on the other end of the clamp. Tubes can be attached in combination with a pump to provide a constant air flow and/or gas exchange.

# Instrument Action Indicator

The Action Indicator has the following:

Indicator Function
Instrument idle and fully charged Instrument is on, and fully charged. The indicator is flashing every 5s
Instrument idle and battery not fully charged Instrument is on, and battery is emptying. The indicator is flashing every 5s
Instrument clamp open Instrument clamp open. The indicator is flashing rapidly.
Instrument busy Instrument is busy (e.g. Measurement). The indicator is constantly on.

# Power/Reset Button

The button in the back allows the instrument to be turned on as well as reset.

  • Hold the button for at least 5 seconds to turn the Instrument on. Wait for the Action Indicator to start flashing, to confirm the Instrument is on and booted up.
  • A short push on the button will reset the instrument, in case a protocol crashes, etc.
  • When the Instrument is running, hold the button for 5 seconds, to restart the instrument. Wait for the Action Indicator to start flashing, to confirm the Instrument is booted up again.


Make sure to only press the power button through the silicon covering.

# Micro-USB

The Micro-USB port allows the Instrument to be charged and when connected to a computer for data transfer. When unplugging the cable, make sure not to bend the cable up and down, to not break of the Instrument connector.

# Accessory Port

The USB-C (opens new window) port allows you to attach accessory equipment. When unplugging the cable, make sure not to bend the cable up and down, to not break of the Instrument connector.

# Charge Indicator

The little LED in the back indicates when the Instrument is charging. When the battery is fully charged, the LED will turn off.

# Configuration

MultispeQ v2.0 measurements
Body Measurements
Size 224.2 mm x 53.6 mm x 56.6 mm (l, w, h)
Total Weight 340 g (incl. battery)
Light Guide Surface 8 mm x 8 mm (64 mm²)
Leaf Clamp Opening 13 mm
Safety Ring for leash
Updates Firmware updates through Applications

# Lights

There are sets of five LEDs each on the main body and the leaf clamp.

# Main Body

# Emission Peak Model Specifications
1 530nm LED (green), Lumileds, LXZ1-PM01 view (opens new window)
2 655nm LED (red), Lumileds, LXZ1-PA01 view (opens new window)
3 590nm LED (amber), Lumileds, LXZ1-PL01 view (opens new window)
4 448nm LED (blue), Lumileds, LXZ1-PR01 view (opens new window)
5 950nm LED (NIR), OSRAM, SFH 4441 view (opens new window)

# Leaf Clamp

# Emission Peak Model Specifications
6 950nm LED (NIR), OSRAM, SFH 4441 view (opens new window)
7 655nm LED (red), Lumileds, LXZ1-PA01 view (opens new window)
8 850nm LED (NIR), OSRAM, SFH 4451 view (opens new window)
9 730nm LED (far red), Everlight, ELSH-Q61F1-0LPNM-JF3F8 view (opens new window)
10 820nm LED (NIR), OSRAM SFH 4786S view (opens new window)

# Emission Spectra for LEDs

Emission spectra for LEDs build in the MultispeQ v2.0 - Emission normalized to maximum emission peak

# Detectors

The MultispeQ v2.0 comes with two detectors. One covering the near infrared on the main body, the other covering the visual range on the leaf clamp.

# Detection Range Model Specifications
1 700nm - 1150nm Hamamatsu, S6775-01 (main body) view (opens new window)
3* 400nm - 700nm Hamamatsu, S6775 (leaf clamp) view (opens new window)


The detector 3 is covered with a BG-18 bandpass filter with a center wavelength around 493 nm. If you are using the detector to detect signals on the edges of the detection range, expect the signal strength to be low.

# Sensors

The MultispeQ v2.0 has a set of sensors to measure the environmental parameters.

Sensor Model Specifications
Ambient Temperature, Humidity, Pressure (2x) BOSCH, BME280 view (opens new window)
Contactless Temperature Melexis, MLX90615SSG-DAG-000-TU view (opens new window)
Accelerometer Freescale, MMA8653FCR1 view (opens new window)
Magnetometer Freescale, MAG3110FCR1 view (opens new window)
Hall Effect Sensor TT Electronics, OHS3150U view (opens new window)
PAR light sensor AMS-TAOS USA, TCS34715FN + 700nm low pass filter view (opens new window)

# Filters

The MultispeQ v2.0 has two filters, a Band Pass filter covering the visible light detector (#3) and a Low Pass filter used in the PAR sensor.

Filter Model Specifications
Band Pass Schott, BG-18 1mm thickness view (opens new window)
Low Pass UQC Optics, Hot Mirror HM-07 view (opens new window)

# Indicator Lights

The MultispeQ v2.0 has now two indicator lights. One to indicate if the device is charging which is located next to the micro-USB port. The second one is located on top to indicate progress or required actions.

LED Model Specifications
Charging Indicator ---
Progress/Action Indicator NeoPixel (RGB), SK6812 view (opens new window)

# Battery

The MultispeQ v2.0 has a Li-ion battery which can be charged through the micro USB port.

Battery Model Specifications
Main Soshine Li-ion 26650 Protected Battery: 5500mAh 3.7V view (opens new window)

# References

Kuhlgert, S., Austic, G., Zegarac, R. Osei-Bonsu, I.,Hoh, D., Chilvers, M. I., et al. (2016). MultispeQ Beta: a tool for large-scale plant phenotyping connected to the open PhotosynQ network. R. Soc. Open Sci. 3, 160592. doi:10.1098/rsos.160592 (opens new window).