MultispeQ v2.0 Calibration

Before getting started with the calibration, make sure you followed all the steps listed in the Overview.

Calibration Assistants

Assistant	Description
Complete Recalibration	Complete recalibration of the MultispeQ Instrument including the PAR Sensor, the LEDs of the main body and the Leaf Clamp, the Leaf Thickness Gauge, Relative Chlorophyll (SPAD), the Internal Compass and the Leaf Clamp's Open/Close position. Requirements: □ CaliQ Instrument □ Chlorophyll Calibration Cards □ Fluorescent Card (pink) □ Thickness Calibration Cards
Open & Close Positions	Set the open and close position of the Leaf Clamp. This becomes important when using the start protocol on open and/or close functions. This is not for calibrating the Leaf Thickness Gauge.
LEDs	Recalibrate the Instrument’s LEDs to match the set light intensities defined in measurement protocols. LEDs with an emission peak between 400nm and 700nm are calibrated to output intensities in PAR. Requirements: □ CaliQ Instrument □ Chlorophyll Calibration Cards □ Fluorescent Card (pink)
PAR Sensor	Calibrate the Instrument's PAR sensor. Requirements: □ CaliQ Instrument
Leaf Thickness	Calibrate the Instrument's Leaf Thickness Gauge. Requirements: □ Thickness Calibration Cards
Relative Chlorophyll (SPAD)	Calibrate the MultispeQ for relative Chlorophyll measurements (Minolta SPAD). Requirements: □ Chlorophyll Calibration Cards

Manual Calibrations

Note

Make sure to follow the steps in the correct order when a calibration contains multiple steps. The same is the case for steps within a calibration routine.

Calibrate PAR Sensor

Calibrate PAR Sensor	Description	Details	Firmware
Step 1: Calibrate PAR Sensor	Follow the instructions prompted by the protocol.	view (opens new window)	>=2.34

When using the CaliQ for calibrating the PAR sensor, make sure it is connected and communicating before starting the calibration procedure. To check if it is properly connected, go to Instrument Settings (opens new window) and test the connection.

Note: These calibration steps require a CaliQ

Calibrate LEDs

Calibrate LEDs	Description	Details	Firmware
Step 1: Main Body LEDs	Follow the instructions prompted by the protocol. Make sure the CaliQ sensor has a snug fit.	view (opens new window)	>=2.34
Step 2: Leaf Clamp LEDs	Follow the instructions prompted by the protocol. Make sure the CaliQ sensor has a snug fit.	view (opens new window)	>=2.34
Step 3: IR LEDs	Follow the instructions prompted by the protocol.	view (opens new window)	>=2.34

Note: These calibration steps require a CaliQ

Electronic Offsets

Electronic Offsets	Description	Details	Firmware
Step 1: Detector Offsets	Follow the instructions prompted by the protocol.	view (opens new window)	>=2.34
Step 2: Detector Offsets - Fluorescence	Follow the instructions prompted by the protocol.	view (opens new window)	>=2.34

Note: These calibration steps require the chlorophyll calibration and the pink fluorescent cards

Calibrate Leaf Thickness

Calibrate Leaf Thickness	Description	Details	Firmware
Step 1: Leaf Thickness Gauge	Follow the instructions prompted by the protocol. Make sure the r² value is 0.98 or higher. If it is not the case, repeat the calibration.	view (opens new window)	>=2.34

Note: These calibration steps require the thickness calibration cards

Calibrate Relative Chlorophyll

Calibrate Relative Chlorophyll	Description	Details	Firmware
Step 1: Relative Chlorophyll (SPAD)	Follow the instructions prompted by the protocol. Make sure the panels of the calibration cards are properly clamped.	view (opens new window)	>=2.34

Note: These calibration steps require the chlorophyll calibration cards

Reset Instrument

Reset Instrument	Description	Details	Firmware
Reset to Default	Reset the MultispeQ to its default settings and remove all calibrations	view (opens new window)	>=2.34

Note: After this step you must finish all further recalibration steps to use the MultispeQ!